Studies on Sucrose-Induced Autolysis of Clostridial Cells Part 3. Autolytic Formation of Clostridial Protoplasts (Autoplasts) as Revealed by Microscopy and Their Some Properties
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چکیده
When exponentially growing cells of Clostridium saccharoperbutylacetonicum (ATCC 13564) were exposed to hypertonic concentration of sucrose (0.3-o. 5 M), rapid degradation of the cell wall occurred (sucrose-induced autolysis). The morphological changes from the original rod-shaped cells to protoplast-like cells during the sucrose-induced autolysis were observed by phase contrast and electron microscopy. When the cells were autolysed in the sucrose solution (0.35M), each cell began to swell at the middle or at one pole and then formed a small bulb at the swollen part. The bulb gradually enlarged as lysis progressed, and finally became a protoplast-like cell (PLC) which had no cell wall. The larger pre-division cell formed the bulb at the middle (septal site), while the small post-division cell formed the bulb at the pole. PLCs had the fo l lowing proper t ies : (1) they were osmot ica l ly fragile and liberated cytoplasmic components into the suspending fluid by osmotic shock; (2) they were effectively stabilized by 0.4M sucrose so lu t ion conta in ing 5 mM Mg2+ ; (3) they could not adsorb phage particles, but they were infected by phenol-extracted phage DNA; (4) they, as same as parent (normal) cells, produced an inducible bacteriocin (clostocin 0) after treatment with mitomycin C; (5) they were able to grow volumentrically and became larger by continuous cultivation in stabilizing liquid medium, but were unable to multiply; and (6) they synthesized all their macromolecules (DNA, RNA, protein and lipid), but DNA synthetic ability became weak as cultivation progressed. From these results, they were regarded as living protoplasts (autoplasts).
منابع مشابه
Studies on Sucrose-Induced Autolysis of Clostridial Cells Part 1. Induction of a Rapid Bacterial Autolysis by Sucrose Treatment
Growing cells of Clostridium saccharoperbutylacetonicum Nl-4 (ATCC 13564) were rapidly autolyzed in the presence of 0.3 to 0.5M sucrose. This autolysis was apparently induced by the treatment with sucrose, and termed “sucrose-induced autolysis.” The maximum effect was shown at 0.35M sucrose, exhib i t ing about 50% loss of initial turbidity of the cell suspension. The rate of lysis depended on ...
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